Anti-Cancer Antigen 125 (CA 125) Antibody

Supplied by Antibodies.com, this Mouse monoclonal antibody targets Cancer Antigen 125 (CA 125), the MUC-16 mucin widely used as a serological marker for ovarian carcinoma and related epithelial tumors. The reagent is produced as an ion-exchange purified monoclonal antibody from the Sp2/0-Ag14 myeloma line and is offered unconjugated in a 1 mg format suitable for high-volume assay work. It is validated for ELISA, Western blotting (WB), and immunohistochemistry (IHC), with reported reactivity against the human CA 125 / MUC-16 protein. The immunoglobulin subclass is IgG (IgG2a family), and the host species is Mouse, ensuring a single, well-defined epitope recognition rather than the polyclonal heterogeneity seen in polyclonal reagents. Storage recommendations are consistent with standard Antibodies.com primary antibody formats: short-term hold at 2–8 °C in the supplied buffer and long-term storage below –20 °C, avoiding repeated freeze–thaw cycles to preserve binding activity. Researchers using the antibody for IHC typically titrate starting around 5–10 µg/mL on formalin-fixed paraffin-embedded tissue, while ELISA and Western blot users commonly dilute into the 1–2 µg/mL working range; precise optimal dilutions should be determined empirically for each detection system. As an ovarian cancer-related tumor marker, CA 125 (Mucin-16) detection supports studies in tumor biology, biomarker validation, and diagnostic assay development.