Anti-Calpain 5 Antibody

Calpain 5 (CAPN5) is a calcium-dependent, non-lysosomal cysteine protease expressed across multiple human tissues, with notable involvement in retinal homeostasis and inflammatory signaling pathways. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and validated for three orthogonal detection workflows: Western blot analysis of cell and tissue lysates, immunohistochemistry on fixed sections, and immunofluorescence imaging.

The 100 µl format is supplied at working dilution ranges suitable for standard blotting membrane and slide-based protocols, and reactivity is confirmed against the human target. CAPN5 research frequently centers on autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV), a rare ocular disorder in which gain-of-function variants drive complement dysregulation, retinal vasculopathy, and progressive vision loss, making reliable isoform-specific detection tools essential for mechanistic studies. Researchers investigating protease activity, calcium-regulated signaling cascades, and retinal disease models use this reagent alongside matched secondary antibodies to localize expression patterns and track protein-level changes in patient-derived samples, induced pluripotent stem cell-derived retinal organoids, and experimental disease systems.