Anti-Calnexin (phospho-S583) Antibody

Calnexin phospho-S583 antibody from Antibodies.com is a rabbit polyclonal reagent raised against a phosphorylated epitope on the C-terminal cytosolic tail of the endoplasmic reticulum (ER) chaperone calnexin. Phosphorylation at serine 583 has been linked to ER stress signaling and the unfolded protein response, and investigators studying these pathways use this reagent to track activation status in human, mouse, and rat samples.

The antibody is supplied as 100 µl of liquid at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2. For research use only, it should be handled refrigerated, and aliquots are recommended to minimize freeze-thaw cycles of the working stock; long-term storage at -20 degrees C is suitable for the aliquoted material.

Validated applications include Western blot and immunohistochemistry, enabling detection of phosphorylated calnexin in cell lysates and in fixed tissue sections. The unconjugated format gives laboratories flexibility to choose secondary detection reagents and chromogenic or fluorescence workflows appropriate to the chosen assay.