Anti-Calnexin Antibody

Supplied by Antibodies.com, this well-characterized polyclonal reagent provides reliable detection of the endoplasmic reticulum chaperone Calnexin across a broad range of sample types. Raised in rabbit, the affinity-purified Ig preparation is offered in a 50 µl volume at a working concentration of 1 mg/mL, formulated in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide for long-term stability. The antibody was generated against a synthetic peptide derived from the human Calnexin sequence in the region surrounding the non-phosphorylation site of serine 583, and it specifically recognizes endogenous levels of the target protein. Researchers can apply it in Western blotting, immunohistochemistry, immunocytochemistry/immunofluorescence, and ELISA workflows, with validated reactivity against human, mouse, and rat specimens, making it a versatile loading-control or ER-marker option for comparative studies. The product is unconjugated, allowing flexible pairing with secondary reporters of the user's choice, and the polyclonal format increases the likelihood of consistent recognition even if minor sequence variation exists between species. For best results, the vial should be stored at –20 °C after aliquoting, with repeated freeze/thaw cycles avoided to preserve binding activity. This research-use-only reagent is intended for laboratory applications such as membrane-bound and secreted protein analysis, antigen presentation studies, and ER-stress investigations.