Anti-Calnexin Antibody

Calnexin is a calcium-dependent lectin chaperone anchored in the endoplasmic reticulum membrane, where it transiently associates with nascent N-glycosylated polypeptides to support correct folding and retention of misfolded intermediates. This unconjugated rabbit polyclonal reagent is raised against the Calnexin target and is validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows.

From Antibodies.com, the antibody is supplied as 100 µl of liquid and is reactive with human, mouse, and rat samples. Because the host is rabbit and the clonality is polyclonal, multiple epitopes on the ER-resident chaperone are recognized, which helps when probing conserved regions or comparing expression across tissues. The unconjugated format leaves secondary label and detection chemistry choices to the researcher.

Common uses include tracking ER membrane content in cell lysates, confirming organelle fractionation purity by Western blot, and visualizing endoplasmic reticulum architecture in fixed cells by immunofluorescence. For handling, refrigerate the vial on receipt and avoid repeated freeze-thaw cycles to preserve activity. The reagent is intended for research use only.