Anti-CALML5 Antibody

Affinity purified from rabbit serum using a recombinant fusion protein corresponding to amino acids 1–146 of human CALML5 (NCBI RefSeq NP_059118.2), this polyclonal primary antibody from Antibodies.com detects the 16 kDa calmodulin-like protein 5 isoform. Each 50 µl vial is supplied as an unconjugated IgG in PBS pH 7.30 with 0.02% sodium azide and 50% glycerol, leaving the reagent available for pairing with HRP-, biotin-, or fluorophore-conjugated secondary antibodies chosen to match each lab's detection chemistry.

For Western blot, the antibody is titrated between 1:500 and 1:2000, giving a predicted band near the 16 kDa marker; for immunohistochemistry on formalin-fixed, paraffin-embedded tissue, working dilutions of 1:50 to 1:200 have been used. The polyclonal format is intentional in IHC, where multiple epitopes improve signal recovery after antigen retrieval. Reactivity has been characterized on human, mouse, and rat samples, supporting side-by-side comparisons of epidermal and mucosal staining across species. CALML5, also called calmodulin-like skin protein (CLSP), is a four EF-hand calcium-binding partner of transglutaminase 3 in differentiating keratinocytes and is widely tracked in psoriasis, atopic dermatitis, and squamous carcinoma tissue panels.

Refrigerate the stock upon arrival, freeze in small working aliquots if it will not be consumed quickly, and protect from repeated freeze-thaw cycles to preserve titer. Centrifube briefly before opening to recover the full volume. Researchers investigating keratinocyte differentiation, epidermal barrier biology, and epithelial tumor expression profiles use this reagent to map CALML5 localization in skin, oral mucosa, and other stratified squamous epithelia.