Anti-CALML5 Antibody

Raised in rabbit against a recombinant fusion protein corresponding to amino acids 1-146 of human CALML5 (NCBI reference NP_059118.2), this polyclonal IgG reagent from Antibodies.com is affinity purified and supplied in a 100 µl vial as an unconjugated liquid. The expected molecular weight of the target is approximately 16 kDa, consistent with the small EF-hand calcium-sensor protein, and the reagent is shipped in phosphate-buffered saline at pH 7.30 with 0.02% sodium azide and 50% glycerol as a cryoprotectant.

Recommended working dilutions are 1:500 to 1:2000 for Western blot and 1:50 to 1:200 for immunohistochemistry on formalin-fixed paraffin-embedded sections, validated against human, mouse, and rat samples. The polyclonal format provides multi-epitope recognition, useful when probing low-abundance calcium-binding proteins in tissue lysates and in chromogenic staining of differentiating keratinocytes, where CALML5 partners with transglutaminase 3 during the terminal differentiation program of stratified squamous epithelia.

Store at -20 °C and limit freeze-thaw cycles by aliquoting on first use to preserve antibody titer for reproducible band detection and immunostaining across experiments. Research applications supported by this reagent include calcium-binding studies, immunoblotting of A431 lysates, and staining of epidermal sheets in psoriasis susceptibility and skin barrier investigations.