Anti-Caldesmon (S783) Antibody

This rabbit polyclonal antibody from Antibodies.com selectively recognizes caldesmon phosphorylated at serine 783 (S783), a modification regulated through ERK/MAPK signaling in smooth muscle and vascular tissues. Raised in rabbit and supplied as an unconjugated reagent at 1 mg/ml in phosphate buffered saline (pH 7.2) with 0.05% sodium azide, the 100 µl volume supports multiple experimental runs across immunoblotting, immunohistochemistry, and immunofluorescence workflows.

Caldesmon is an actin- and myosin-binding protein that governs actomyosin ATPase activity and thin-filament stability. Phosphorylation at S783 alters its interaction with actin and is implicated in smooth muscle phenotype switching, cytoskeletal remodeling during cell migration, and altered signaling in certain tumor contexts. The reagent has been validated on human, mouse, and rat samples, enabling cross-species comparative studies in cardiovascular, gastrointestinal, and oncology research.

Recommended applications include western blot detection of phosphorylated caldesmon in cell and tissue lysates, immunohistochemistry on formalin-fixed sections to localize S783 phosphorylation in vascular and visceral smooth muscle layers, and immunofluorescence for subcellular distribution studies. For long-term use, store frozen in working aliquots to minimize freeze-thaw cycles and preserve epitope recognition. Sodium azide at 0.05% prevents microbial contamination during routine handling.