Anti-CAD Antibody

Targeting the caspase-activated DNase, this unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the CAD protein and validated for Western blot and immunohistochemistry workflows on human samples. CAD catalyzes internucleosomal DNA cleavage during apoptosis and operates as the active subunit of the heterodimeric DNA fragmentation factor, where it remains bound and inhibited by ICAD (DFF45) until caspase-3 cleaves the inhibitor to release CAD's nuclease activity. Polyclonal sera recognize multiple epitopes on the target, supporting detection of the full-length protein in lysates and tissue sections. The 100 µl aliquot is supplied as an unconjugated preparation, allowing laboratories to couple it to their preferred reporter or to use it directly in chromogenic and fluorescence workflows. Researchers studying programmed cell death, DNA damage response, and tumor biology rely on this reagent to localize CAD expression across fixed tissue sections and to confirm expression levels by immunoblotting.