Anti-C1R Antibody - Identical to Abcam (ab205546)

This Rabbit polyclonal antibody from Antibodies.com targets complement component C1r, the serine protease that initiates the classical complement pathway by cleaving C1s. Supplied as an IgG preparation at 100 µl and unconjugated, the reagent is validated for Western blot and immunofluorescence workflows on human, mouse, and rat samples.

The polyclonal format provides multi-epitope recognition, which helps when individual linear epitopes are masked by SDS denaturation or aldehyde-based fixation. Researchers using Western blot typically resolve C1r near its predicted molecular weight in reduced whole-cell or tissue lysates, while immunofluorescence on paraformaldehyde-fixed cells and cryosections localizes the protease at sites of classical pathway engagement. Suggested starting dilutions are commonly in the 1:500 to 1:2,000 range for Western blot and 1:100 to 1:500 for immunofluorescence; downstream titers should be optimized per assay.

Aliquot upon receipt to limit freeze-thaw cycles, store frozen at -20 °C or colder, and protect from light when handling fluorophore-conjugated secondary reagents. Each vial contains 100 µl of antibody. This product is for research use only and is not intended for diagnostic or therapeutic applications.