Anti-C1D Antibody

C1D is a small, highly basic ~16 kDa SAP-domain nuclear protein of 141 amino acids implicated in DNA double-strand break repair, telomere protection through TRF2 interaction, 3′ end processing of preribosomal RNA, and RNA polymerase I transcription, making the rabbit polyclonal Anti-C1D reagent from Antibodies.com a targeted tool for chromatin, ribosome biogenesis, and V(D)J recombination studies. The antiserum is raised against recombinant full-length human C1D and affinity-purified by chromatography against the epitope-specific immunogen, supplied at approximately 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as 100 µl in a vial. On western blots of whole-cell lysates, the unconjugated IgG produces the predicted ~16 kDa band together with the SDS-resistant ~32 kDa homodimer reported for endogenous C1D in human fibroblasts, while on fixed cells immunofluorescence reveals discrete nuclear C1D foci that intensify following DNA damage induction. The antibody is validated for western blot and immunofluorescence across human, mouse, and rat samples, and storage at -20°C with working aliquots kept at 4°C preserves activity across repeated freeze-thaw cycles. For research use only.