Anti-c-Src (T523) Antibody

c-Src is a non-receptor tyrosine kinase that plays central roles in cell proliferation, survival, adhesion, and migration signaling cascades. Antibodies.com offers this rabbit polyclonal reagent that specifically recognizes the phosphorylated T523 residue of human c-Src, enabling researchers to interrogate kinase activation states in both cell and tissue contexts. The antibody is supplied as a 50 µl aliquot of 1 mg/ml total protein in phosphate buffered saline with 15 mM sodium azide at approximately pH 7.2, and is unconjugated for flexible assay development.

Validated for Western blot and immunohistochemistry workflows, the antibody demonstrates cross-reactivity with mouse and rat orthologs alongside human samples, supporting comparative studies across standard laboratory models. The polyclonal format increases the likelihood of robust signal even when target expression is low, and the PBS-based formulation is compatible with common blocking and wash buffers. Researchers studying Src family kinase signaling, focal adhesion biology, or oncogenic transformation pathways frequently rely on phosphorylation-state-specific reagents such as this one to dissect upstream regulators and downstream effectors of c-Src activity.