Anti-c-Src (phospho-Y529) Antibody

This rabbit polyclonal reagent from Antibodies.com selectively detects c-Src phosphorylated at tyrosine 529, the C-terminal negative regulatory site whose modification by CSK-family kinases locks the kinase in an autoinhibited, closed conformation. Provided at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, the 100 µl format supports Western blot and immunohistochemistry workflows on human, mouse, and rat samples. Investigators use this unconjugated affinity-purified antibody to monitor Src family kinase inactivation state in receptor signaling studies, tumor and metastasis models, focal adhesion turnover, and neuronal development, where the balance between inhibitory Y529 phosphorylation and activating Y419 autophosphorylation is a common readout.