Anti-c-Src Antibody

This polyclonal primary antibody from Antibodies.com targets c-Src, a non-receptor protein tyrosine kinase encoded by the SRC proto-oncogene. The reagent is raised in rabbit and supplied as 100 µl of unconjugated antiserum, validated for Western blot (WB), immunohistochemistry (IHC), and ELISA workflows on human, mouse, and rat samples.

c-Src belongs to the Src family of kinases (SFKs) and operates downstream of receptor tyrosine kinases, integrins, and G-protein-coupled receptors, modulating proliferation, cytoskeletal dynamics, and adhesion. Researchers studying breast carcinoma, colorectal cancer, and other epithelial malignancies routinely probe Src signaling by immunoblotting for total c-Src alongside phospho-Y419. The antibody is also used in neuroscience, osteoclast biology, and immune-cell signaling, where Src family activity shapes synaptic plasticity, bone resorption, and lymphocyte activation.

Because the product is unconjugated, it pairs readily with HRP-, biotin-, or fluorophore-conjugated secondary antibodies matched to the detection platform. For Western blot, whole-cell or tissue lysates are resolved by SDS-PAGE, transferred to nitrocellulose or PVDF, and probed at dilutions guided by lot-specific titration data. For IHC on formalin-fixed paraffin-embedded sections, heat-induced epitope retrieval in citrate buffer (pH 6.0) is recommended prior to overnight primary incubation in a humidified chamber. Aliquot upon first use and store at -20 degrees C in working volumes to limit freeze-thaw cycles and preserve binding activity across downstream applications.