Anti-c-Src Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this polyclonal reagent from Antibodies.com targets the endogenous c-Src protein and is supplied as a 50 µL liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody is unconjugated and was generated against a synthesized peptide derived from human c-Src around the non-phosphorylation site of Y419, making it well suited to detect the native kinase in samples of human, mouse and rat origin. Validated applications include ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF) and immunohistochemistry (IHC), so the reagent can be deployed across blotting, imaging and assay-based workflows in a single laboratory. The antibody is shipped at 4°C and should be aliquoted upon arrival and stored at -20°C, with repeated freeze/thaw cycles avoided to preserve binding activity. Intended for research use only, this Antibodies.com primary antibody offers a versatile tool for signalling studies focused on the Src family of non-receptor tyrosine kinases, with specificity for detecting endogenous c-Src levels in a range of cell- and tissue-based experimental systems.