Anti-c-Jun (phospho-T239) Antibody

Phosphorylation of the c-Jun transcription factor at threonine 239 is a regulatory event linked to DNA-binding activity and AP-1 driven gene expression. Antibodies.com supplies this affinity-purified rabbit polyclonal reagent as an unconjugated IgG preparation at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, supplied as 100 µl per vial. The antibody recognizes the phosphorylated T239 epitope on human and mouse c-Jun, making it suitable for western blot and immunofluorescence workflows that probe stress-activated kinase signaling, mitogenic responses, and AP-1 transcriptional output. Investigators studying UV-induced DNA damage, growth factor pathways, or oncogenic transformation can apply the reagent to immunoblotting of whole-cell lysates or to visualization of nuclear c-Jun in fixed cells. The polyclonal format yields consistent detection across denaturing gels and supports visualization of the modified epitope in chromatin-associated fractions. Storage at -20 °C in working aliquots is recommended to limit freeze-thaw cycles of the glycerol-containing formulation, while routine bench handling at 4 °C is supported by the sodium azide preservative. The unconjugated format allows users to couple the antibody to HRP, fluorophores, or biotin depending on the chosen detection chemistry.