Anti-c-Jun Antibody

From the Antibodies.com primary antibody portfolio, this Anti-c-Jun reagent (catalog A32407, supplied through AFMS in Saudi Arabia) is a mouse monoclonal antibody raised against recombinant full-length human c-Jun and supplied as 50 µl of purified immunoglobulin in 0.1 M Tris-Glycine buffer (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody is unconjugated, produced from mouse ascites and affinity-purified by affinity chromatography on the immunogen, with purity greater than 95% by SDS-PAGE. It is recommended for Western blot at a working dilution of 1:1000, detecting endogenous levels of c-Jun (a 36 kDa AP-1 transcription factor subunit encoded by JUN) with an observed band at approximately 45 kDa, and shows no cross-reactivity with related Jun family proteins. Reactivity has been validated on Human and Mouse samples, making the reagent suitable for studying AP-1-driven transcriptional regulation, Jun/Jun homodimer and Jun/Fos heterodimer formation, and signalling downstream of phorbol ester tumour promoter response elements. For optimal stability, the supplier recommends short-term storage at 4 °C with aliquoting and long-term storage at -20 °C, avoiding repeated freeze-thaw cycles, and the product is intended for research use only, not for diagnostic or therapeutic procedures. Priced at 2275.0 SAR through AFMS, this c-Jun monoclonal offers a reliable, validated tool for western blot workflows investigating oncogenic transformation and stress-activated transcription factor activity.