Anti-c-Fms (E557) Antibody

Raised in rabbit against a peptide epitope in the intracellular domain of c-Fms (also known as CSF1R, the colony-stimulating factor 1 receptor), this polyclonal antibody from Antibodies.com detects a receptor tyrosine kinase implicated in monocyte and macrophage lineage commitment, osteoclastogenesis, and tumor-associated microglial signaling. The immunogen region centers on residue E557, a conserved site within the kinase domain that has been used in published characterizations of ligand-induced receptor activation and receptor internalization studies.

Validated for Western blot, immunohistochemistry, and immunofluorescence workflows, the reagent cross-reacts with human, mouse, and rat samples, supporting comparative studies across species and tissue types including bone marrow, placenta, and tumor sections. It is supplied as 50 µl of affinity-purified antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide, pH approximately 7.2. The unconjugated format allows flexible pairing with custom fluorophore, biotin, or HRP conjugates depending on the chosen detection chemistry.

For routine handling, keep the vial refrigerated and protect from light; avoid repeated freeze-thaw cycles by aliquoting on first use. Researchers working on c-Fms/CSF1R biology, microglial activation models, or receptor tyrosine kinase signaling can deploy this reagent for expression profiling, phosphorylation analyses, and subcellular localization studies in fixed and frozen tissue preparations.