Anti-BUB3 Antibody

This affinity-purified reagent from Antibodies.com is a rabbit polyclonal antibody raised against recombinant human BUB3, also known as mitotic checkpoint protein BUB3. It is supplied at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, and is offered in a 50 µl format suitable for routine bench use. The antibody has been validated for Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) applications, and it shows reactivity with human, mouse and rat samples, making it a flexible choice for cell-cycle and checkpoint research across the most common model systems. Being unconjugated and polyclonal, the reagent allows convenient pairing with a wide range of labelled secondary antibodies of the researcher's choice. The immunogen is a recombinant human BUB3 protein, and the antibody is purified by affinity chromatography using that immunogen to maximise specificity for endogenous BUB3. For handling and storage, the product is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with repeated freeze/thaw cycles avoided to preserve activity. Intended for research use only, this reagent is a reliable option for laboratories studying spindle assembly checkpoint signalling, chromosome segregation and mitotic fidelity.