Anti-BUB1 Antibody

Rabbit polyclonal antibody from Antibodies.com targeting BUB1, a mitotic checkpoint serine/threonine kinase that localizes to kinetochores during early mitosis and enforces the spindle assembly checkpoint to ensure accurate chromosome segregation. This unconjugated reagent is supplied as 100 µl aliquots and is validated for Western blot (WB) and immunohistochemistry (IHC) applications on human samples. BUB1 has been studied extensively in the context of aneuploidy and tumor progression, where its overexpression has been documented across multiple cancer types including colorectal, gastric, and breast carcinomas. The antibody recognizes epitopes on the full-length BUB1 protein, supporting detection of endogenous kinase levels in cell lysates by WB and localization patterns in fixed tissue sections by IHC. Researchers investigating mitotic fidelity, kinetochore signaling, or cancer cell biology can use this reagent to monitor BUB1 expression alongside related effectors such as BUBR1, CENP-E, and Aurora B.