Anti-BUB1 Antibody

This unconjugated rabbit polyclonal reagent targets BUB1, a mitotic checkpoint kinase that localizes to unattached kinetochores and helps enforce the spindle assembly checkpoint during cell division. Raised in rabbit and affinity-purified, the antibody from Antibodies.com is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a stabilizer, packaged as a 100 µl vial. Validated for Western blot and immunohistochemistry on human and mouse samples, it supports studies of chromosome segregation fidelity, kinetochore signaling, and mitotic checkpoint control in cancer cell lines and tissue sections. The polyclonal format provides recognition of multiple BUB1 epitopes, which is frequently investigated in the context of aneuploidy, tumor progression, and cellular responses to antimitotic drugs. Refrigerate on arrival; for longer-term storage, aliquot and freeze, avoiding repeated freeze-thaw cycles to preserve binding activity. The unconjugated format gives researchers flexibility to conjugate the antibody to fluorophores, biotin, or enzymes for custom detection workflows in cell cycle and oncology research.