Anti-BUB1 Antibody

Rabbit polyclonal antibody to BUB1, supplied by Antibodies.com as an unconjugated IgG reagent raised in rabbit. The catalogue listing indicates validation for Western blot and immunohistochemistry, with reactivity against human, mouse, and rat samples.

Manufacturer specifications for the same product list Western blot and ELISA as validated applications, with reactivity against human and mouse samples. The antibody was raised against a recombinant fusion protein corresponding to amino acids 1–300 of human Bub1.

It is affinity purified and supplied in liquid form in phosphate-buffered saline (pH 7.3) with 50% glycerol and 0.02% sodium azide. The predicted molecular weight of the target is 122 kDa, and a Western blot dilution range of 1:500–1:1,000 is recommended. The reagent is shipped at 4°C and should be aliquoted and stored at −20°C, with freeze/thaw cycles avoided.