Anti-Btk (phospho Y223) Antibody

This phospho-specific primary antibody recognizes Bruton's tyrosine kinase at phosphorylated tyrosine 223, enabling researchers to examine Btk activation rather than total Btk abundance. Antibodies.com supplies the antibody as an unconjugated rabbit polyclonal IgG for western blot and immunofluorescence applications, with listed reactivity against human, mouse, and rat samples.

Provided in a 50 µl pack, the reagent is useful for western blot analysis of phospho-Btk in stimulated or treated cell lysates and for immunofluorescence visualization of phosphorylated Btk in fixed cells. Fluorescently or enzymatically labeled secondary antibodies can be selected according to the detection platform. Appropriate unstimulated and stimulated controls are important because phosphorylation-dependent signal can change rapidly with cellular conditions. Sample preparation, antibody dilution, and detection conditions should be optimized for each experimental system. For research use only.