Anti-BTK Antibody [Assigned #A0500]

This unconjugated mouse monoclonal antibody from Antibodies.com targets Bruton's tyrosine kinase (BTK), a cytoplasmic Tec-family kinase essential for B-cell receptor signaling, B-cell maturation, and innate immune responses. Clone A0500 is supplied as a 200 µl liquid aliquot of IgG and is validated for Western blot and immunoprecipitation workflows on human and mouse samples.

Researchers commonly deploy the reagent to probe BTK expression and phosphorylation status in B-cell lymphoma lines, primary lymphocyte lysates, and models of X-linked agammaglobulinemia, where BTK loss-of-function variants abrogate signaling downstream of the pre-B-cell receptor. Because the antibody is unconjugated, it pairs cleanly with HRP-, fluorophore-, or biotin-labeled secondary reagents selected to match the chosen detection platform.

Aliquot upon receipt and store refrigerated to preserve activity across multiple freeze-thaw cycles; handle lysates on ice during immunoprecipitation to limit kinase dephosphorylation. For research use only, not intended for diagnostic or therapeutic applications.