Anti-BTK Antibody [Assigned #A0500]

This mouse monoclonal IgG reagent from Antibodies.com targets Bruton's tyrosine kinase (BTK), a cytoplasmic non-receptor tyrosine kinase that operates downstream of the B-cell receptor to govern B-cell maturation and antigen-receptor signal transduction. Supplied as 100 µl of unconjugated antibody and validated for Western blot (WB) and immunoprecipitation (IP), the clone recognizes BTK from both human and mouse sources, making it suitable for comparative immunology studies and signal-transduction research.

BTK occupies a critical position in the pre-BCR and BCR signaling cascades, where it phosphorylates BLNK and PLCγ2 to relay membrane-proximal signals to NF-κB. Loss-of-function mutations in the BTK gene produce X-linked agammaglobulinemia, while dysregulated BTK activity is implicated in B-cell malignancies including chronic lymphocytic leukemia and mantle cell lymphoma. The BTK pathway is the pharmacological target of covalent inhibitors such as ibrutinib, acalabrutinib, and zanubrutinib, so the antibody supports kinase biology research, drug-target validation, and analysis of B-cell receptor pathway inhibition in lymphoma cell lines and primary samples.

For laboratory workflow, the antibody should be aliquoted into working volumes to limit freeze-thaw cycles, with refrigerated short-term storage between uses. The unconjugated format offers flexibility to pair the antibody with secondary reagents such as HRP, biotin, or fluorophore conjugates, and supports kinase phosphorylation studies, co-immunoprecipitation of BTK-containing signaling complexes, and Western blot detection of BTK expression across B-cell developmental stages and lymphoma models.