Anti-Btk Antibody

Raised in rabbit and supplied as 100 µl of unconjugated antiserum, the Btk polyclonal antibody from Antibodies.com is validated for western blotting, immunohistochemistry, and ELISA. Bruton's tyrosine kinase is a Tec family non-receptor kinase that transmits signals downstream of the B-cell receptor and contributes to innate immune pathways involving TLR and Fc receptor activation. Because the reagent recognizes Btk epitopes conserved across human, mouse, rat, and monkey samples, it supports comparative studies of B-cell development, X-linked agammaglobulinemia, and the kinase's role in chronic lymphocytic leukemia, mantle cell lymphoma, and other B-cell malignancies where covalent Btk inhibitors such as ibrutinib are under active clinical evaluation.

The unconjugated format gives laboratories flexibility to pair the primary with HRP, fluorescent, or biotinylated secondary reagents of their own choosing, making the antibody suitable for western blot detection of endogenous Btk in lymphocyte lysates, IHC localization in spleen and lymph node sections, and ELISA-based readouts of BCR pathway activity.