Anti-BTD Antibody

From Antibodies.com, this rabbit polyclonal antibody targets human biotinidase (BTD), the lysosomal enzyme responsible for recycling biotin from biocytin and other biotinylated degradation products. Supplied as 100 µl of liquid at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated IgG fraction is validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human-derived samples. Biotinidase is widely expressed in hepatic and renal tissue, where the antibody enables detection of endogenous BTD by immunoblotting, while the IHC application supports localization studies in formalin-fixed paraffin-embedded sections. The polyclonal format delivers epitope diversity for robust recognition of native and denatured target, and the glycerol-containing buffer allows convenient storage at -20 °C without freezing the entire aliquot, with 0.1% sodium azide preserving microbial stability for routine benchtop use. Researchers studying inherited biotinidase deficiency, biotin metabolism, or hepatic protein processing can use this reagent to probe endogenous BTD levels in cell lysates and tissue sections.