Anti-BTAF1 Antibody

BTAF1 (B-TFIID TATA-box binding protein associated factor 1) is the largest subunit of the human TFIID transcription complex and the functional ortholog of yeast Mot1, acting as a DNA-dependent ATPase that remodels TBP-promoter contacts during RNA polymerase II initiation. This rabbit polyclonal antibody from Antibodies.com is supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, unconjugated and validated for Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation (ChIP) in human, mouse, and rat samples.

The polyclonal format delivers broader epitope coverage than monoclonal reagents, which is advantageous when probing conformational variants, post-translational modifications, or degradation intermediates of the ~200 kDa BTAF1 protein in nuclear extracts and fixed tissue. Store refrigerated in the supplied glycerol buffer; aliquot on first use and avoid repeated freeze-thaw cycles to preserve binding activity. Investigators working on chromatin biology, transcriptional regulation, or oncogenic transcription programs use BTAF1 reagents to dissect how TBP dynamics shape promoter selectivity across the genome.