Anti-BrdU Antibody

Manufactured by Antibodies.com and distributed through Bio-Connect, this unconjugated primary antibody is supplied as a mouse monoclonal reagent raised against 5-bromo-2′-deoxyuridine (BrdU), the thymidine analogue widely used to label cells traversing S-phase. The antibody is presented at 0.5 mg/ml in a lyophilized format using PBS at pH 7.4 with 0.02% sodium azide as a preservative, and the 50 µl pack size is suitable for routine proliferation and cell-cycle studies. The product is validated for three application areas: Flow Cytometry, Immunofluorescence, and ELISA, giving laboratories flexibility for both surface/intracellular staining and quantitative detection of incorporated nucleoside. Purity is reported at >95% and the reagent is certified for Research Use Only, not for diagnostic or therapeutic applications. As a monoclonal reagent produced in mouse, the antibody offers batch-to-batch consistency for tracking newly synthesised DNA in pulse-labelling experiments, with the unconjugated format allowing researchers to couple it to their preferred fluorophore or enzyme. Lyophilized preparations of this type are typically reconstituted in sterile buffer shortly before use and stored at 2–8 °C short term or −20 °C long term, avoiding repeated freeze–thaw cycles to preserve activity.