Anti-BrdU Antibody

Bromodeoxyuridine (BrdU) is a synthetic thymidine analog that gets incorporated into newly synthesized DNA during the S-phase of the cell cycle, making it a reliable marker for tracking actively dividing cells in research applications. This unconjugated mouse monoclonal reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, presented in a 100 µl format suitable for multiple staining experiments across a busy lab schedule.

Validated for immunohistochemistry, immunofluorescence, and flow cytometry, the antibody enables detection of proliferating cells in fixed tissue sections, cultured cell preparations, and suspension samples. BrdU labeling protocols typically require DNA denaturation via acid or heat treatment to expose the incorporated nucleoside for antibody binding, after which primary antibody incubation followed by appropriate secondary detection reagents produces clear nuclear staining patterns confined to S-phase cells.

Researchers routinely use BrdU detection to quantify cell proliferation rates in neuroscience, tumor biology, developmental studies, and regenerative medicine investigations. For long-term storage the reagent should be kept refrigerated and protected from light, with aliquots recommended to minimize freeze-thaw cycles that can degrade antibody performance over time, and the 1 mg/ml concentration allows convenient dilution for individual assay needs.