Anti-BRCA1 Antibody

This rabbit polyclonal reagent from Antibodies.com targets the BRCA1 tumor suppressor, a key regulator of homologous recombination repair and DNA double-strand break signaling. Raised in rabbit and supplied as 100 µl of unconjugated antiserum in a single vial, the antibody is validated for western blot and immunohistochemistry workflows. Researchers studying hereditary breast and ovarian cancer susceptibility routinely use it to probe BRCA1 expression in patient-derived tumor sections and to monitor protein abundance in cell lysates from engineered cell lines. The polyclonal format recognizes multiple epitopes on the full-length BRCA1 protein, which is advantageous when investigating truncation variants or post-translational modifications. For handling, store the vial at -20 °C in working aliquots to limit freeze-thaw cycles and protect from light, allowing the reagent to equilibrate on ice before dilution into blocking buffer. Reactivity has been confirmed against human BRCA1, making the reagent useful for translational studies with human cell lines and clinical tissue samples, while the 100 µl volume supports both small pilot experiments and larger screening panels.