Anti-BRCA1 Antibody

Offered through Antibodies.com, this polyclonal reagent is a rabbit-derived IgG preparation raised against recombinant full-length human BRCA1, enabling sensitive detection of endogenous BRCA1 protein in standard immunoassays. The antibody is supplied at 50 µl per vial, formulated at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a stabilizer, and is unconjugated, allowing researchers to couple it to their preferred label or detection system. It is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications and has been shown to react with Human, Mouse, and Rat samples, making it a versatile choice for cross-species studies of DNA damage response and tumor biology. Affinity-purified by epitope-specific chromatography, the preparation is reported to exceed 95% purity by SDS-PAGE, and the target migrates at the expected ~200 kDa molecular weight. Synonymous names include Breast cancer type 1 susceptibility protein, RING finger protein 53, RNF53, and RING-type E3 ubiquitin transferase BRCA1, reflecting its dual role as a tumor suppressor and E3 ubiquitin ligase. Suitable secondary antibodies such as Goat Anti-Rabbit IgG H&L conjugates (AP, Biotin, FITC, and HRP) are listed on the product page for convenient workflow completion. Intended for research use only and not for diagnostic or therapeutic applications, this reagent provides a reliable tool for laboratories investigating BRCA1 function, hereditary breast and ovarian cancer mechanisms, and broader chromatin-associated signaling pathways.