Anti-BRCA1 Antibody

This polyclonal antibody from Antibodies.com targets the BRCA1 tumor suppressor protein, a 1863-amino-acid nuclear phosphoprotein of approximately 220 kDa that is central to homologous recombination-mediated DNA double-strand break repair and the maintenance of genomic stability. Raised in rabbit, the reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotective storage buffer, packaged as a 100 µl vial of unconjugated antibody.

Validated applications include western blot detection of endogenous BRCA1 from cell lysates, immunohistochemistry on paraffin-embedded tissue sections, and immunofluorescence staining of nuclear foci that form at sites of DNA damage following genotoxic stress. The antibody has been confirmed to react with BRCA1 derived from human, mouse, and rat samples, making it a practical reagent for comparative oncology studies across model organisms and patient-derived tumor specimens.

For storage, the glycerol-containing formulation keeps the antibody stable at -20°C between uses, with aliquoting recommended to limit repeated freeze-thaw cycles that can erode antigen-binding activity. Researchers investigating hereditary breast and ovarian cancer biology, PARP inhibitor response mechanisms, or BRCA1/BARD1 complex assembly will find this reagent adaptable to standard chromogenic and fluorescence-based detection protocols in research use only settings.