Anti-BRCA1 (Ab-1423) Antibody

Offered through Antibodies.com, this affinity-purified reagent is a rabbit polyclonal reagent raised against a synthetic peptide corresponding to amino acids 1421–1425 (H-G-S-Q-P) of the human BRCA1 protein, enabling sensitive detection of endogenous total BRCA1 in research samples. The unconjugated antibody is supplied as a 50 µL aliquot at 1.0 mg/mL in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. Recommended working dilutions are 1:500–1:1000 for Western blotting and 1:50–1:100 for immunohistochemistry, validated for human reactivity with a predicted molecular weight of approximately 220 kDa. Polyclonal antibodies were generated by immunizing rabbits with the BRCA1 peptide–KLH conjugate and purified by affinity chromatography on an epitope-specific peptide column, ensuring high specificity toward the target sequence surrounding residue 1423. The product is intended for research use, ships with a typical lead time of 6–9 business days, and is part of the Antibodies.com primary antibody catalogue, making it a reliable tool for laboratories investigating DNA damage response, tumor suppression pathways, and hereditary breast cancer research.