Anti-BRCA1 (Ab-1423) Antibody

BRCA1 phosphorylation at Ser1423 is a hallmark of ATM/ATR kinase activation during the cellular DNA damage response. This rabbit polyclonal reagent from Antibodies.com specifically recognizes the epitope region surrounding residue 1423 of the human breast cancer susceptibility protein, enabling researchers to monitor DNA damage signaling events in cell-based and tissue-based assays.

The unconjugated antibody is supplied as a 100 µl liquid aliquot and is validated for Western blot (WB) and immunohistochemistry (IHC) on human samples, including formalin-fixed paraffin-embedded tissue sections. It supports detection of endogenous BRCA1 in cell lysates and tumor specimens, providing a reliable tool for studies of homologous recombination repair, PARP inhibitor sensitivity, and hereditary breast and ovarian cancer research. Standard reducing SDS-PAGE conditions are recommended for Western blot, while citrate-based antigen retrieval is recommended for IHC.