Anti-BRAF35 Antibody

This rabbit polyclonal reagent from Antibodies.com targets BRAF35, also referred to as HMG20B, a high-mobility-group DNA-binding protein that partners with the LSD1-CoREST histone demethylase complex in chromatin remodeling and transcription regulation.

The antibody is supplied as 100 µL of unconjugated antiserum raised in rabbit and is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA against human samples. BRAF35 expression is most often studied in the context of neuronal differentiation and chromatin-modifying complexes, so researchers use this reagent to probe LSD1 pathway components, track co-localization with partner proteins such as REST and LSD1 in fixed cells and tissue sections, and follow changes in chromatin-associated fractions across differentiation time courses.

The polyclonal format broadens epitope coverage across the target, which is useful when probing conserved HMG-box domains or modified forms of BRAF35. For working use, small aliquots are recommended to limit freeze-thaw cycles of the 100 µL stock, and the unconjugated format gives users flexibility to conjugate the antibody to a reporter of choice for custom detection assays.