Anti-BNIP3L Antibody

BNIP3L (also known as NIX) is an outer mitochondrial membrane protein belonging to the BH3-only subfamily of BCL-2 family members, acting as a key effector of selective mitophagy and apoptosis regulation. This unconjugated polyclonal reagent from Antibodies.com is raised in rabbit and cross-reacts with human, mouse, and rat samples, supporting comparative work across standard mammalian model systems.

Validated applications include Western blot for endogenous protein detection and immunohistochemistry for tissue localization of mitophagy markers. The antibody is supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, an anti-freeze formulation that tolerates repeated freeze-thaw cycles when stored at -20°C. Working aliquots should be kept refrigerated and used promptly to preserve activity. For Western blot, expected band sizes appear around 28-35 kDa depending on isoform and post-translational modification status.

BNIP3L functions as a receptor for LC3/GABARAP family proteins on the mitochondrial surface, recruiting autophagosomal machinery to depolarized mitochondria during programmed mitochondrial clearance. Investigators studying hypoxia signaling, Parkin-independent mitophagy, reticulocyte maturation, and cardiac or neural stress responses commonly use this reagent to monitor BNIP3L expression and mitochondrial turnover dynamics in cell culture and tissue samples.