Anti-BNIP3 Antibody

The BNIP3 target is a Bcl-2 family pro-apoptotic protein anchored in the outer mitochondrial membrane, where it governs programmed cell death and selective mitophagy under hypoxic and oxidative stress conditions. This rabbit polyclonal reagent from Antibodies.com is raised against the human BNIP3 sequence and detects the endogenous protein in human and mouse samples by western blot and immunohistochemistry on formalin-fixed tissue sections.

Supplied as an unconjugated 100 µl aliquot of rabbit antiserum-derived IgG, the antibody is useful for investigators working on hypoxia signaling, mitochondrial turnover, cardiac ischemia-reperfusion injury, and tumor cell survival pathways. The polyclonal format broadens epitope coverage, which is valuable when probing post-translationally modified forms of the protein that arise during autophagy induction.

For western blot, dilute in blocking buffer and incubate overnight at 4 °C; for immunohistochemistry, perform antigen retrieval in citrate buffer (pH 6.0) prior to standard chromogenic detection. Working dilutions may be kept briefly at 4 °C; for longer storage, freeze the reagent and limit freeze-thaw cycles to preserve activity.