Anti-BNIP-3 Antibody

BNIP3 is a pro-apoptotic member of the Bcl-2 protein family that anchors in the outer mitochondrial membrane and drives programmed cell death under hypoxic and ischemic stress. This Antibodies.com reagent is a rabbit polyclonal antibody raised against BNIP3, supplied as an unconjugated preparation of 100 µL and validated for Western blot and ELISA workflows on human samples.

The antibody is commonly used in studies of mitophagy, tumor hypoxia signaling, and cardiac ischemia-reperfusion injury, where researchers probe mitochondrial fractions and total cell lysates to monitor changes in BNIP3 expression alongside related effectors such as BNIP3L/Nix and Beclin-1. Its polyclonal profile supports recognition of multiple epitopes on the target, and the unconjugated format offers flexible pairing with HRP, fluorescent, or biotin-labeled secondary reagents of the researcher's choice.

Investigators working on autophagy induction, mitochondrial turnover, or programmed necrosis pathways apply this antibody to track BNIP3 abundance in cultured human cell lines and primary tissue lysates under different stress conditions, including hypoxia mimetics and nutrient withdrawal.