Anti-BMAL1 Antibody

Offered by Antibodies.com as a primary antibody reagent, this rabbit polyclonal reagent targets the BMAL1 transcription factor (also known as ARNTL), a core component of the mammalian circadian clock machinery. The product is supplied as a 50 µl liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide, and it is unconjugated for direct detection workflows. The antibody has been affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen corresponding to a synthesized peptide derived from human BMAL1 around the non-acetylation site of K538, and it specifically detects endogenous levels of BMAL1 protein only when not acetylated at K538. Validated applications include Western blot (WB) and ELISA, with reactivity confirmed against human, mouse, and rat samples, making it suitable for cross-species circadian rhythm, chronobiology, and metabolic regulation studies. Researchers can therefore use this reagent to probe BMAL1 expression in cell lysates and tissue homogenates across standard immunoblotting and immunoassay protocols, with the polyclonal nature of the antibody providing broad epitope coverage for robust signal detection.