Anti-BIRC5 Antibody [Assigned #A10738]

This rabbit monoclonal reagent from Antibodies.com is raised against BIRC5, the 16.5 kDa inhibitor-of-apoptosis protein commonly referred to as Survivin. Produced in rabbit as an IgG isotype and supplied unconjugated in a 200 µl liquid format, the preparation is qualified for Western blot, immunohistochemistry, and immunoprecipitation workflows on mouse and rat samples. The antibody targets an epitope on BIRC5, supporting studies of mitotic regulation, apoptosis resistance, and tumor cell survival where Survivin expression serves as both a marker and a mechanistic variable. Researchers commonly pair this reagent with nuclear and cytoplasmic fractionation to map the dual localization of Survivin in proliferating cells, and it has been applied to cultured mammalian lines as well as fixed tissue sections. Routine handling includes brief equilibration to room temperature before use, aliquoting to limit freeze-thaw cycles, and refrigerated storage of the stock vial protected from light. As an unconjugated primary, the reagent is readily adaptable to a secondary detection system of choice and fits into broad immunodetection pipelines, including Western blot of whole-cell lysates, IHC on formalin-fixed paraffin-embedded sections, and IP for co-precipitation experiments with binding partners of Survivin such as Aurora B kinase and XIAP.