Anti-BIRC2 Antibody

This rabbit polyclonal reagent from Antibodies.com targets BIRC2, a member of the inhibitor of apoptosis (IAP) protein family also known as cIAP1. BIRC2 functions as a ubiquitin E3 ligase and a key regulator of TNF receptor and NF-κB signaling, making it relevant to studies of programmed cell death, innate immune responses, and tumor cell survival. The antibody is supplied as 100 µl of liquid at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, with the unconjugated format allowing flexible pairing with chromogenic, fluorescent, or HRP-conjugated secondary antibodies.

Validated reactivity covers human, mouse, and rat samples, supporting both basic research and translational workflows in oncology and inflammation biology where dysregulated IAP signaling is a recurring theme. The polyclonal format provides multi-epitope recognition across full-length cellular extracts and tissue sections, which is useful when investigating post-translationally modified forms of BIRC2. Recommended applications listed for this antibody include Western blot, immunohistochemistry, and immunofluorescence, enabling detection across denatured lysates, fixed tissue, and intact cellular preparations.

For routine laboratory handling, store the vial refrigerated between uses and aliquot upon first thaw to limit repeated freeze-thaw cycles, which preserves binding activity across multiple experiments. Sodium azide in the buffer serves as a microbial preservative for working stocks, while the 50% glycerol component provides cryoprotection for storage at lower temperatures. Researchers studying cIAP1 involvement in apoptotic regulation, inflammatory cascade activation, or ubiquitin ligase biology can integrate this reagent into standard immunodetection pipelines with appropriate species-matched secondary antibodies.