Anti-BID (phospho-S78) Antibody

This rabbit polyclonal antibody from Antibodies.com selectively recognizes the BH3-only Bcl-2 family member BID when phosphorylated at serine 78, an ATM-dependent modification generated in cells experiencing DNA double-strand breaks. The reagent is supplied at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, in a 100 µl volume, and is unconjugated so it can be paired with secondary reagents of the researcher's choice. It has been validated for Western blot and immunohistochemistry and shows reactivity with human, mouse, and rat samples, allowing studies that compare cell line data with primary tissue.

Investigators working on the intrinsic apoptosis pathway, DNA damage response signaling, and the crosstalk between ATM kinase and p53 tumor suppressor networks use phospho-S78 BID as a marker of pathway engagement rather than a simple readout of total protein. The polyclonal format increases tolerance for conformational variation around the modified epitope, which is useful when probing full-length BID alongside truncated or cleaved tBID species that arise during apoptosis. Sodium azide in the formulation limits microbial growth during routine bench use, and aliquoting is recommended to minimize freeze-thaw cycles that would otherwise erode binding activity over extended storage.