Anti-beta Actin Antibody

From Antibodies.com, this mouse monoclonal Anti-beta Actin antibody recognizes the 42 kDa cytoplasmic β-actin isoform that serves as a widely used loading control for normalizing protein input in Western blot experiments. The reagent is supplied as a liquid IgG1 preparation at 100 µg, unconjugated, and validated across immunoprecipitation, immunofluorescence, immunohistochemistry, and ELISA workflows. Demonstrated reactivity spans human, mouse, rat, bovine, sheep, porcine, rabbit, feline, canine, guinea pig, chicken, carp, and leech, supporting comparative work between mammalian and non-mammalian species. For long-term storage, aliquot the antibody and freeze at -20°C; limit freeze-thaw cycles to protect binding activity. On standard SDS-PAGE gels, the antibody produces a discrete band near 42 kDa in whole-cell lysates and tissue homogenates, making it useful as an internal reference for signal quantification.