Anti-beta Actin Antibody

This rabbit polyclonal IgG antibody from Antibodies.com targets beta Actin (ACTB), a highly conserved 42 kDa cytoskeletal protein routinely used as a loading control in western blot workflows. Raised in rabbit and supplied as an unconjugated liquid preparation at 100 µg per vial, the reagent is validated for reactivity against human, mouse, rat, monkey, and fruit fly samples, supporting normalization across diverse cell line and tissue lysates. Recommended working dilutions for western blot generally fall between 1:500 and 1:2000 with HRP-conjugated secondary antibodies and chemiluminescent detection. Store aliquots frozen at -20 °C in single-use vials and limit freeze-thaw cycles to preserve antibody activity; avoid prolonged exposure to room temperature.