Anti-BCOR Antibody

Raised in rabbit against the BCL6 Corepressor (BCOR) antigen, this unconjugated polyclonal IgG reagent from Antibodies.com is supplied as a 50 µl liquid aliquot for Western blot applications. The target itself is a ubiquitously expressed transcriptional regulator that forms the scaffold of the non-canonical Polycomb Repressive Complex 1.1, recruiting histone H2A mono-ubiquitination activity to specific genomic loci.

BCOR loss-of-function and translocation events are recurrent drivers in acute myeloid leukemia, clear cell sarcoma of the kidney, and dedifferentiated endometrial stromal sarcoma, so immunodetection of the endogenous protein is a routine tool in cancer research and developmental biology pipelines. This preparation has been validated for detection of the native protein in lysates derived from human, mouse, and rat samples, giving cross-species compatibility for studies that compare BCOR expression across cell line panels and primary tissue from multiple model organisms.

The polyclonal format offers multiple epitope recognition, which is advantageous when probing full-length BCOR alongside truncated or mutant isoforms generated by leukemia-associated translocations such as the ZC3H7B-BCOR fusion. Recommended use begins with standard WB sample preparation in RIPA buffer, separation by SDS-PAGE, and transfer to nitrocellulose or PVDF membrane, followed by an HRP- or fluorophore-conjugated secondary reagent for chemiluminescent or fluorescence-based imaging workflows.