Anti-Bcl-X Antibody

The Bcl-X protein is a member of the Bcl-2 family and a central regulator of mitochondrial apoptosis, with the anti-apoptotic Bcl-xL isoform and the pro-apoptotic Bcl-xS splice variant both derived from the BCL2L1 gene. This rabbit polyclonal reagent from Antibodies.com was raised against Bcl-X and validated for Western blot and immunohistochemistry workflows, enabling detection of endogenous Bcl-xL and Bcl-xS expression in tissue sections and whole-cell lysates. The antibody cross-reacts with Human, Mouse, and Rat samples, supporting studies in cancer cell lines, primary tumor tissue, neuronal survival models, and rodent disease models where altered apoptosis signaling is under investigation.

Supplied as 100 µl of unconjugated antibody at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the product is shipped in a single-use vial suitable for immediate dilution into standard blocking buffer or antibody diluent. The polyclonal format provides multi-epitope recognition, which is useful when probing isoform-specific banding patterns, cleavage products, or post-translational modifications of Bcl-X across apoptosis time-course experiments and IHC staining of formalin-fixed sections.