Anti-Bcl-w Antibody

The Bcl-w protein is an anti-apoptotic member of the Bcl-2 family encoded by the BCL2L2 gene, where it regulates mitochondrial outer membrane permeabilization and intrinsic apoptosis signaling. This unconjugated rabbit polyclonal reagent from Antibodies.com targets Bcl-w and is validated across Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. Confirmed reactivity against human, mouse, and rat samples supports apoptosis, cancer biology, and neurodegeneration research where Bcl-w regulation, isoform expression, or compensatory function within the broader Bcl-2 network is of interest.

Supplied as a 100 µl liquid aliquot of unconjugated antiserum, the antibody offers flexibility for downstream labelling or direct detection. Polyclonal reactivity broadens epitope coverage along the Bcl-w sequence, an advantage when probing endogenous protein from tissue lysates, primary cultures, or fixed sections. Standard Western blot workflows resolve Bcl-w near its expected molecular weight using reducing SDS-PAGE followed by chemiluminescent or fluorescent secondary antibodies. For IHC and IF, the reagent is applied to paraformaldehyde-fixed cells or cryosections to map subcellular distribution, including the mitochondrial localisation patterns characteristic of Bcl-2 family biology.

For handling, refrigerate the stock upon arrival, protect from light, and prepare single-use aliquots to limit freeze-thaw cycles so titre is preserved across multi-week experiments. Short-term bench handling on wet ice is acceptable during protocol setup, and brief room temperature exposure during pipetting will not materially affect performance when standard aseptic technique is observed.