Anti-Bcl-2 (phospho-T69) Antibody

Phosphorylation of the anti-apoptotic regulator Bcl-2 at threonine 69 is a post-translational modification linked to cellular stress responses, growth factor withdrawal, and genotoxic damage, and it modulates the protein's survival function in a manner distinct from total Bcl-2 expression levels. This rabbit polyclonal antibody from Antibodies.com was raised against a phospho-T69-containing immunogen and selectively detects Bcl-2 only when threonine 69 is phosphorylated, enabling researchers to resolve the active signaling fraction from the broader Bcl-2 pool in apoptosis and cell survival studies.

The reagent is supplied as a 100 µl volume at 1 mg/ml in phosphate buffered saline (PBS) containing 15 mM sodium azide at approximately pH 7.2, and is validated for Western blot and immunohistochemistry applications across human, mouse, and rat samples. For long-term storage, keep the vial frozen at -20 °C in single-use working aliquots to minimize freeze-thaw cycles, since phospho-specific epitopes are vulnerable to dephosphorylation and conformational drift on repeated thawing. The unconjugated format gives flexibility to pair the primary with HRP-, Alexa Fluor-, or biotin-conjugated secondary reagents depending on the chosen detection platform, supporting work in autophagy, cancer cell signaling, and chemoresistance research.