Anti-Bcl-2 Antibody

Raised in rabbit against the B-cell lymphoma 2 (Bcl-2) protein, this polyclonal primary antibody from Antibodies.com is supplied as 100 µl of unconjugated serum and is validated for Western blot, immunohistochemistry, and ELISA workflows. Bcl-2 is the founding member of the Bcl-2 family of apoptosis regulators and acts primarily at the mitochondrial outer membrane to block caspase activation, making its expression a common readout in studies of tumor cell survival, chemoresistance, and neurodegeneration.

The reagent reacts with human and monkey samples, so it bridges routine clinical specimens and non-human primate model work without requiring a separate antibody. Because the host species is rabbit, it pairs cleanly with the most commonly available secondary reagents (anti-rabbit HRP, biotin, or Alexa Fluor conjugates), and the unconjugated format leaves labeling choices to the end user. Polyclonal preparations also tend to recognize multiple epitopes on the target, which is helpful when probing Bcl-2 across isoforms or in samples where post-translational modification may mask individual linear epitopes.

Typical applications include probing whole-cell or mitochondrial lysates by Western blot to compare Bcl-2 levels in tumor versus normal tissue, staining formalin-fixed paraffin-embedded sections by immunohistochemistry to localize expression to specific cell populations, and adapting the antibody for plate-based quantitative assays. Researchers studying follicular lymphoma, breast carcinoma, and other malignancies where Bcl-2 is overexpressed frequently use this reagent alongside pro-apoptotic family members (Bax, Bak, Bad) to map the balance between survival and death signaling in the same experimental system.